学者简介以及论文摘要
M.E.诺海森教授:
马来西亚普特拉大学健康科学专业和学生会副系主任、马来西亚普特拉大学营养和饮食系讲师。
在过去20年里，她发表或合作发表80多篇有关文章。其中有：2017年美国转化研究杂志刊登“ 咖啡酸苯乙酯可防止硫酸钠诱发的实验性溃疡结肠炎”。2017年医学档案刊登“肌醇六磷酸抑制mTOR通路和诱导自噬-在HT-29结肠癌细胞介导的细胞死亡”。目前还是多个课题研究生的主要导师。
Sustained Release of Anticancer Agent Phytic Acid From Its Chitosan-Coated Magnetic Nanoparticles for Drug Delivery System
Norhaizan ME, Farahnaz B, Dena D, Bullo S, Sivapragasam G, Mohd Zohir H, Ashok Kumar P and Palanisamy A
Faculty of Medicine and Health Sciences, Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia (UPM), 43400 Serdang Selangor, MALAYSIA
Extensive studies using chitosan (CS)-iron oxide magnetic nanoparticles (MNPs) nanocarrier have been performed recently to enhance anti-cancer drug efficacy and reduce the side effects of the drug. Phytic acid (PA) which is found abundantly in plant and legumes has been reported to possess a broad range of pharmaceutical properties including as anticancer agent. However, it has a very short plasma half-life and is released very fast. So, the aim of this research is to synthesize a new form of nanohybrid phytic acid-chitosan-MNPs and explore the potential usage in the delivery of anti-cancer PA. The resulting nanocomposite and nanocarrier will be characterized by powder X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometry (VSM), transmission electron microscopy (TEM) and thermogravimetric and differential thermogravimetric (TGA/DTG) analysis. FTIR spectra and thermal analysis for the iron oxide magnetic nanoparticles (MNPs) and PA-CS-MNP nanocomposite has confirmed the binding of chitosan (CS) on the surface of MNP nanoparticles and the loading of PA in the PA-CS-MNP nanocomposite. The coating process enhanced the thermal stability of phytic acid in the nanocomposite and the X-ray diffraction results showed that the MNPs, and PA-CS-MNP nanocomposite are pure magnetite. The drug loading was estimated using ultraviolet–visible spectroscopy and showing a 12.9% in the designed nanocomposite. Magnetization curves demonstrated that the synthesized MNPs and nanocomposite were superparamagnetic with saturation magnetizations of 53.25emu/g and 42.15emu/g, respectively. The release study showed that around 86% and 93% of PA from PA-CS-MNP nanocomposite could be released within about 127 and 56 hours by a phosphate buffer solution at pH 7.4 and 4.8, respectively which was found to be in sustained manner and governed by pseudo-second order kinetic model. The cytotoxicity of the compounds on HT-29 colon cancer cell were also evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The HT-29 cell line is more sensitive against PA-CS-MNP nanocomposite than phytic acid alone. No cytotoxic effect was observed on normal cells (3T3 fibroblast cells) which indicates that PA-CS-MNP nanocomposite is able to inhibit the proliferation of colon cancer cells without causing any harmful effect on normal cell.
壳聚糖包裹磁性纳米微粒给药系统的抗癌药植酸的缓释作用
——M.E. 诺海森教授 马来西亚普特拉大学
应用壳聚糖（CS）-氧化铁磁性纳米粒子（MNPS）纳米载体所做的大量研究在近期已经完成，来提高抗癌药物疗效、降低药物的副作用。植酸（PA）在植物和豆科植物中大量存在，据报道它具有广泛的药用特性，包括作为抗癌剂。然而，它的血浆半衰期很短，并且释放得非常快。因此，本研究的目的是合成一种新的纳米植酸壳聚糖磁性颗粒复合物以及探索抗癌PA输送中的潜在用途。所取得的纳米复合材料和纳米载体将通过粉状X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、振动样品磁力测定（VSM），透射电子显微镜（TEM）和热重差热（TGA / DTG）分析得以显现。对氧化铁磁性纳米颗粒(MNPs)和PA-CS-MNP进行傅里叶变换红外光谱（FTIR）和热分析证实MNP纳米粒子表面壳聚糖（CS）的粘合以及PA-CS-MNP中PA的加载。涂层工艺使纳米复合材料中的植酸耐热性得到增强，而X射线衍射结果也表明MNP和PA-CS-MNP纳米复合材料属于纯磁铁矿。用紫外-可见光谱估算载药量在设计的纳米复合材料中占比显示为12.9%。磁化曲线表明合成磁性纳米材料的纳米复合材料的超顺磁性饱和磁化强度分别为与53.25emu/g和42.15emu/g。现有的研究表明，PA和PA-CS-MNP介于86%和93%的纳米复合物可在127小时到56小时之中于浓度为pH 7.4和4.8的磷酸盐缓冲溶液中释放，分别被发现呈持续状态，并受伪二阶动力学模型控制。使用3,4,5-三羟基苯甲酸二聚体3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide（MTT）检测法对HT-29结肠癌细胞的化合物的毒性进行了评估。HT-29细胞株对PA-CS-MNP复合物比对单独植酸更敏感。在正常细胞中没有观察到细胞毒作用（3T3成纤维细胞），这表明PA-CS-MNP纳米复合材料能够抑制结肠癌细胞的增殖，对正常细胞没有造成任何不良影响。
